| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201782 |
| Chromosome: | chromosome 2 |
| Location: | 1409543 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083400 | (1 of 1) PF07000 - Protein of unknown function (DUF1308) (DUF1308) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAAAAGGAAACAGCAGCTGTGTGTGTG |
| Internal bar code: | CTAGTCTGGCCTTAGCGTTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 1300 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCATGCAAACAGACAC |
| Suggested primer 2: | GTGGAGGTGAAGAACCAGGA |