| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201783 |
| Chromosome: | chromosome 2 |
| Location: | 4210186 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCTCCTTTGCCTCTGGACCTTTCCCA |
| Internal bar code: | TATGGATAGGCGGCTACTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 98.7212 |
| LEAP-Seq n confirming: | 386 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACTTCGAGCTGCCCAC |
| Suggested primer 2: | AACTGACAAACAAGCCTCGG |