| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201835 |
| Chromosome: | chromosome_10 |
| Location: | 4097975 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g449750 | PKS1 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTCCCCCACCCACCCGCGCGCCCGCCCC |
| Internal bar code: | TAAGACCTTACAAGGTAGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 40.5941 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGAGAGGCATACACAAA |
| Suggested primer 2: | CATAGGCCACAATGGGTAGG |