| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201845 |
| Chromosome: | chromosome 4 |
| Location: | 2664209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223100 | CAH1 | (1 of 2) PTHR18952//PTHR18952:SF104 - CARBONIC ANHYDRASE // CARBONIC ANHYDRASE-RELATED PROTEIN; Carbonic anhydrase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCGCCTACAGGCAAGATCTATCCCCTT |
| Internal bar code: | CCGCGGCGACGGTACGGTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 253 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGTCGTACCCTGACCTG |
| Suggested primer 2: | CACTGACCAGTGACGCAGAT |