| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201861 |
| Chromosome: | chromosome 6 |
| Location: | 5714122 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286750 | (1 of 2) PF15247 - Histone RNA hairpin-binding protein RNA-binding domain (SLBP_RNA_bind) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACCTGATGGAGACACCTGGCCCTGTGT |
| Internal bar code: | TGATTATCCGTCTCCACAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 99.5277 |
| LEAP-Seq n confirming: | 843 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATGTGAGGGAGTGTGG |
| Suggested primer 2: | TGTACAGGCCTCATCAGCAG |