| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201877 |
| Chromosome: | chromosome 4 |
| Location: | 3724989 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229250 | (1 of 1) PTHR10209//PTHR10209:SF100 - OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGCATCGAGGACGGGGGCGGGGTTTG |
| Internal bar code: | CCTGAGCGTCTCCTCATATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 3795 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATGACTCCCAGTTTTCC |
| Suggested primer 2: | CCTTCTTCGAGTGGATCGAG |