| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201892 |
| Chromosome: | chromosome_12 |
| Location: | 4412555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTCTCGGCGCGCGCTGATGGCTGGCACAC |
| Internal bar code: | CACTCACACGCTCTGGGCTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1147 |
| LEAP-Seq percent confirming: | 99.3008 |
| LEAP-Seq n confirming: | 9516 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTCACACTCAGCTCAA |
| Suggested primer 2: | CGACTTCTCCACCTACTCGC |