Insertion junction: LMJ.RY0402.201914_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201914
Chromosome:chromosome 6
Location:8166185
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g305250 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCAATCACCGAGAGATTTGGAGCTTAGT
Internal bar code:GACCCGCTGACTAACGCCTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:90.3361
LEAP-Seq n confirming:215
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGTGACTCACTCTTGTGC
Suggested primer 2:GGTGTCCGTTTCTTTTGCAT