| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201938 |
| Chromosome: | chromosome 2 |
| Location: | 6846082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119450 | (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTAGTAACTCTAGGGTTTCAGCTTACAG |
| Internal bar code: | ATGTTGCCGTCAACTCTTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.8382 |
| LEAP-Seq n confirming: | 617 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCATACGCCACCAGTT |
| Suggested primer 2: | ACACCAAGCCCTACAACGAC |