| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201959 |
| Chromosome: | chromosome 12 |
| Location: | 2183399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508500 | (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGTCGTGAGGCTGGTTAGAGGCAAAC |
| Internal bar code: | CGTGAGCGCTAGACGCCCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 97.7471 |
| LEAP-Seq n confirming: | 1345 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAAACGAGAATTGCGTC |
| Suggested primer 2: | GAAGGTACCACACGAAGGGA |