| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201960 |
| Chromosome: | chromosome 10 |
| Location: | 640612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422050 | (1 of 1) PTHR22763:SF3 - E3 UBIQUITIN-PROTEIN LIGASE RNF115 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCGGCTCTGGACGCGCCTAGGACCTT |
| Internal bar code: | ATCGAAAGTAGTCCTGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.2548 |
| LEAP-Seq n confirming: | 666 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCTTGAAGCCAGACGA |
| Suggested primer 2: | CCTAACGATCCTCCTCCTCC |