| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201964 |
| Chromosome: | chromosome 2 |
| Location: | 1184636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGACGGAGGCGGCAGTAGCGGCGGCGG |
| Internal bar code: | AAGTACCCTGTCCAGGCGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1224 |
| LEAP-Seq percent confirming: | 2.68817 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 362 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCCATTTCCAAGAATC |
| Suggested primer 2: | AGACCACCTGACACAGTCCC |