| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201973 |
| Chromosome: | chromosome 10 |
| Location: | 1239851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426650 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGATAAAGTCCCTCGCGCGTCAGACATTT |
| Internal bar code: | GGAAAGGCCGGGGTGATCGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 99.2021 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGAGCAGTGGTCTGAAG |
| Suggested primer 2: | CAGCGTACACGTCCTCAAAA |