| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201973 |
| Chromosome: | chromosome 16 |
| Location: | 3117743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665600 | CSK7 | (1 of 3) K03097 - casein kinase II subunit alpha (CSNK2A); Casein kinase II-related Ser/Thr kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACTGAAGTCGGGTGGGCTGCCATGGAG |
| Internal bar code: | GTGGAGACGTACGCCGATCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.7034 |
| LEAP-Seq n confirming: | 3361 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACAGTAAGGAGGACCTG |
| Suggested primer 2: | ACCAAAATACTGGCGCAAAC |