Insertion junction: LMJ.RY0402.201979_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201979
Chromosome:chromosome 10
Location:4207531
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g450000 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACCCTTCCGCGGTGCGCCTTCTCCACG
Internal bar code:GCCGCAACAGCTGGCCCAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:810
LEAP-Seq percent confirming:32.1083
LEAP-Seq n confirming:166
LEAP-Seq n nonconfirming:351
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCACCACCTCTCACTCTC
Suggested primer 2:GTCACACACCAAAGACGTGG