| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201980 |
| Chromosome: | chromosome 12 |
| Location: | 1769268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512300 | (1 of 1) 1.13.11.58 - Linoleate 9S-lipoxygenase / Linoleate 9-lipoxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACCATGCAGCACATAGGCCGCAAGACA |
| Internal bar code: | TGTATTCTCGGGTCGTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 88.9197 |
| LEAP-Seq n confirming: | 7696 |
| LEAP-Seq n nonconfirming: | 959 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTTTTCGGCTCTAGTT |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |