| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202028 |
| Chromosome: | chromosome 6 |
| Location: | 7288875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298550 | FMO7 | Flavin-containing monooxygenase; (1 of 3) K00485 - dimethylaniline monooxygenase (N-oxide forming) (FMO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCTTGGGATCCAAGGGGGAAGCAGTTT |
| Internal bar code: | TTACACGGTTGTGTGATTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 98.2063 |
| LEAP-Seq n confirming: | 219 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCCCTAGCCCCTCTACC |
| Suggested primer 2: | GGTCAGCAAACTGAAGGCTC |