| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202089 |
| Chromosome: | chromosome 9 |
| Location: | 5690834 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401478 | (1 of 1) PF03232 - Ubiquinone biosynthesis protein COQ7 (COQ7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAATGGGCGTTGGGCACGCGTGGGCGTT |
| Internal bar code: | ATGAGGCGGGGAGCGCTCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.823 |
| LEAP-Seq n confirming: | 6205 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCCTTGCCTTGTCTGC |
| Suggested primer 2: | CAACATCTGGCCCCACTACT |