Insertion junction: LMJ.RY0402.202097_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.202097
Chromosome:chromosome 3
Location:2639317
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g160900 (1 of 1) PF07714//PF12796//PF13857 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGTGAGAAGGAACGGAAAGGTCATGTAC
Internal bar code:CGGTACGTAGGTCCCCCATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:106
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGACATAGGAGGGGGCAT
Suggested primer 2:GCCAACAGACATACTTCGCA