Insertion junction: LMJ.RY0402.202136_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.202136
Chromosome:chromosome 6
Location:4021116
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278204 (1 of 4) PTHR10334//PTHR10334:SF224 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAAGCCAAAGCACCCCGTGCAACAACT
Internal bar code:CACGCATCAGCATTGTTCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1030
LEAP-Seq percent confirming:99.4213
LEAP-Seq n confirming:4639
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGTTAGTTTGTTGCCCCC
Suggested primer 2:CCTTGTCCTGTTCTGGTGGT