| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202182 |
| Chromosome: | chromosome 1 |
| Location: | 7960572 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g067282 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGTCGGCGAAGTGTTTGCAACCTATC |
| Internal bar code: | TAGGGCTCAATAAGATTGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 16 |
| LEAP-Seq percent confirming: | 99.5316 |
| LEAP-Seq n confirming: | 425 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGAAGGGCAATCAGCG |
| Suggested primer 2: | CGACGAGGATCCACCAAC |