| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.202188 |
| Chromosome: | chromosome 2 |
| Location: | 4352022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099400 | ELG31 | Exostosin-like glycosyltransferase 31; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGTCGGGTTACGGCGGTTAAGTGCAAA |
| Internal bar code: | GCTAGCGGGGTCCCGTATCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 99.1751 |
| LEAP-Seq n confirming: | 1563 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACGATTCCCCTGTCCC |
| Suggested primer 2: | GAGAGAAGGTCAGCCACCAG |