| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202201 |
| Chromosome: | chromosome 16 |
| Location: | 5845020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677700 | NUP62 | Nucleoporin 62; (1 of 1) K14306 - nuclear pore complex protein Nup62 (NUP62, NSP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCACAGGTCCTTACAATACCGGTATC |
| Internal bar code: | AACGAAGATGGTGCTCGGCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 98.8479 |
| LEAP-Seq n confirming: | 858 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAGCACAAATGCAGCGTG |
| Suggested primer 2: | CTCGCACTAGCAGCGTACAG |