Insertion junction: LMJ.RY0402.202216_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.202216
Chromosome:chromosome 13
Location:3196572
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g585500 HAP1 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase; Putative haloperoxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTTGCCACCAAGCTGGGGACAGCTGGG
Internal bar code:TAGCGCGACTGCACGGTCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:871
LEAP-Seq percent confirming:98.4424
LEAP-Seq n confirming:2844
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTCAGGAGTCCGAGAGG
Suggested primer 2:CCACAAGCAGTCAAAGCAAA