| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202241 |
| Chromosome: | chromosome 15 |
| Location: | 804910 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639850 | NCL6,OPR96 | Nuclear Control of chloroplast Like 6, pseudogene; (1 of 45) IPR013584 - RAP domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTATTGCGAGAGTCTAAGAGAGAGCAG |
| Internal bar code: | CCTTACAAGTCCACTTATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.4259 |
| LEAP-Seq n confirming: | 866 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGGTCATCTACGCAT |
| Suggested primer 2: | CTCTACAACCGAAAGCAGCC |