| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202255 |
| Chromosome: | chromosome_2 |
| Location: | 608457 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077850 | FAP212 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCAAAGGATTCATGAAATGTTGCTTGAAT |
| Internal bar code: | GGACATGGCAGTTGACGGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 99.529 |
| LEAP-Seq n confirming: | 6128 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGTGACCAAGGACACCA |
| Suggested primer 2: | ATCCAATTGCCAGGTACAGC |