| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202262 |
| Chromosome: | chromosome 6 |
| Location: | 6499885 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCATTCAACATCATCCGCGCGGACAT |
| Internal bar code: | GGTTGATTGCCGATCATGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.5698 |
| LEAP-Seq n confirming: | 3472 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTAGCTCAGTCAAGGTG |
| Suggested primer 2: | GGGTGTTGAAATGCTTTGCT |