| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202278 |
| Chromosome: | chromosome 12 |
| Location: | 4839449 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524850 | (1 of 1) IPR000104//IPR003593//IPR027417 - Antifreeze protein, type I // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGCAGTGACGTAAGCGCTTCGCCTCT |
| Internal bar code: | AGGTGCCTGGTGTGTGTATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.8576 |
| LEAP-Seq n confirming: | 2104 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCCTCAGGTTTTCGAT |
| Suggested primer 2: | GCGACGGGGAACTTATACAT |