| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202293 |
| Chromosome: | chromosome 2 |
| Location: | 8661118 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142066 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAATTGACGCTACATCGAGCAGTTGCAG |
| Internal bar code: | GCGGAGGAATATTTGTTAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.9172 |
| LEAP-Seq n confirming: | 4826 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAATGCTGGCAAACTGCTG |
| Suggested primer 2: | CCCATTTGTCAGGCTGAAGT |