| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.202329 |
| Chromosome: | chromosome 14 |
| Location: | 269232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609500 | PWR5 | PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAATACAAATACACACACAAACACACC |
| Internal bar code: | TATGTTTTTATCCCTGACTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 98.9744 |
| LEAP-Seq n confirming: | 193 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGTTGTCTTGGGCTTTG |
| Suggested primer 2: | GTCAACATCAATACGGCACG |