| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202339 |
| Chromosome: | chromosome 17 |
| Location: | 336528 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698400 | LCI31 | Low-CO2-inducible protein 31 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACCGACGCACGACCTGCAAAGCCAGG |
| Internal bar code: | TGGCCAATTGTTTCACTGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 99.0695 |
| LEAP-Seq n confirming: | 4791 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACTCGCCGGTTATTGTG |
| Suggested primer 2: | GACGATAGCGCTGTGCATAA |