Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.202351 |
Chromosome: | chromosome_9 |
Location: | 1915282 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g395250 | FAP36 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTTGCAGGGCCGGAAGAGAAATGGGGCAT |
Internal bar code: | TGCCGAAAGACATTAATGGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 192 |
LEAP-Seq percent confirming: | 99.293 |
LEAP-Seq n confirming: | 7303 |
LEAP-Seq n nonconfirming: | 52 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGAGCGATGGGTTTAGTTT |
Suggested primer 2: | GTCTTGTACCTGGTTGCCGT |