| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202374 |
| Chromosome: | chromosome 12 |
| Location: | 8999138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543800 | 3'UTR|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACGTGGGTTCCTGCAGCTTCTACGCT |
| Internal bar code: | ATGAGCTCAGGAGCTTATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 99.8778 |
| LEAP-Seq n confirming: | 817 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGACTCGAGCACCAACG |
| Suggested primer 2: | AGTCCACATTGCCTGCCTAC |