| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202435 |
| Chromosome: | chromosome 2 |
| Location: | 5042673 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104550 | Paf1 complex subunit CDC73; (1 of 1) K15175 - parafibromin (CDC73) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACAGAAATCCATCTAAGCATTCGGCA |
| Internal bar code: | ATGCTGTAGGAAGAAACCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.7563 |
| LEAP-Seq n confirming: | 1228 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCAAACCCCTCACAAAG |
| Suggested primer 2: | CTTAGGTACTGTCTCGCGCC |