| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202439 |
| Chromosome: | chromosome 2 |
| Location: | 1529931 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084450 | PFH10,PHX5,P4H-10 | Prolyl 4-hydroxylase 10; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATGCTGGTCGATCTGGAGTCACTGAGG |
| Internal bar code: | CGAGGGCCGGGGCCCGGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.0598 |
| LEAP-Seq n confirming: | 1475 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTGTGCTAGTTGGTGA |
| Suggested primer 2: | GGGCCAGGTCATAAGTAGCA |