| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202455 |
| Chromosome: | chromosome 2 |
| Location: | 862898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079050 | DGTT5 | Diacylglycerol O-acyltransferase, Type 2; (1 of 4) K14457 - 2-acylglycerol O-acyltransferase 2 (MOGAT2, MGAT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAGCCGTGCCGCCGCCAGCCGCCAGCC |
| Internal bar code: | GCGGTCGCGCGGAGGTGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.2248 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGATAGAAAGGCCCAC |
| Suggested primer 2: | GCTAGTGGGACTTTTGAGCG |