| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202459 |
| Chromosome: | chromosome_16 |
| Location: | 1178251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650600 | MST1 | Mastigoneme-like flagellar protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAACCAAGTTCGTGACGCTTCGCTTGTATG |
| Internal bar code: | GCGAGTCATGGACCGTACGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.8727 |
| LEAP-Seq n confirming: | 2353 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTTCCTGCTATTGGTC |
| Suggested primer 2: | ATCACCACAATCTTCTCCGC |