Insertion junction: LMJ.RY0402.202468_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.202468
Chromosome:chromosome 7
Location:3678093
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g337450 GT90F9,GT90-9,CGL75 Glycosyl transferase GT90 family protein 9; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCCACCCCACCCCACCCCGGCGCCC
Internal bar code:GGCTTTTCCTAGCTTCAGCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:192
LEAP-Seq percent confirming:89.3048
LEAP-Seq n confirming:167
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGTTGGACTGGGAAGTA
Suggested primer 2:TCAAACTGTATCACTGCGCC