Insertion junction: LMJ.RY0402.202468_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.202468
Chromosome:chromosome 7
Location:3678344
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g337450 GT90F9,GT90-9,CGL75 Glycosyl transferase GT90 family protein 9; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGCAGCTGTCCGGCTTGTACAACATTG
Internal bar code:CTGATGGGAACCGTTGGGCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:99.1407
LEAP-Seq n confirming:2192
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTACTTGTCCATGCAGGC
Suggested primer 2:TTTGCGACGTCATTCCATAA