Insertion junction: LMJ.RY0402.202508_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.202508
Chromosome:chromosome 11
Location:492088
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGGCCACCACCCACCCAACCACCCGCT
Internal bar code:GCGAAATAGCCATGGGCACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3
LEAP-Seq percent confirming:57.1142
LEAP-Seq n confirming:285
LEAP-Seq n nonconfirming:214
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTTGGAATCAGCGTCTT
Suggested primer 2:ACCCGTACATCTGCTCAACC