| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202524 |
| Chromosome: | chromosome 16 |
| Location: | 4567701 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688201 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGACACCACCTTTTTGACAGGAAGAC |
| Internal bar code: | TCATCATCCAAAGTAAGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 98.2213 |
| LEAP-Seq n confirming: | 497 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAAGATCCTGGCGCTGA |
| Suggested primer 2: | GTGCCATGGAAGACACATTG |