| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202535 |
| Chromosome: | scaffold 24 |
| Location: | 21282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755247 | (1 of 1) IPR003010//IPR020683 - Carbon-nitrogen hydrolase // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTACAAGTATGCGTACGGCATGGAGGG |
| Internal bar code: | TTTGTCAGATCTTTCATACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.8675 |
| LEAP-Seq n confirming: | 2262 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATCCCTCCAGACCAACCC |
| Suggested primer 2: | TAGCACTTAGCGGAAGCGTT |