| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202573 |
| Chromosome: | chromosome 9 |
| Location: | 4154567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393991 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGTCCTGGTCTCCTGGACCCTCCTAC |
| Internal bar code: | GCGTTTGACGCCTTCTCAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 97.8657 |
| LEAP-Seq n confirming: | 1880 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAGAGGGGAATGCGTA |
| Suggested primer 2: | CTTGTGCCATGGTTGTTGAC |