| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202601 |
| Chromosome: | chromosome 10 |
| Location: | 1623159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429702 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCTTGTAAAGCAGAACAAGAGCGCGCC |
| Internal bar code: | AATAGCCATCTATTGCAAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.557 |
| LEAP-Seq n confirming: | 4045 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGTCGAACTTGCACTCG |
| Suggested primer 2: | GTCGTGATGCAGCTCCTGTA |