| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202613 |
| Chromosome: | chromosome 7 |
| Location: | 4741033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345300 | UAP1 | UDP-N-acetylglucosamine-pyrophosphorylase-related protein; (1 of 1) K00972 - UDP-N-acetylglucosamine/UDP-N-acetylgalactosamine diphosphorylase (UAP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTAGGTGTAGCTGCAGGTGTTGGACGTG |
| Internal bar code: | TTTTGGAGCGTTGAGTACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 186 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTCGTGGTTTTGGTCAG |
| Suggested primer 2: | CTAGAGCCAGTTGAGCCCAC |