| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.202646 |
| Chromosome: | chromosome_9 |
| Location: | 4298580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g394732 | MOT3 | Predicted protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGATGGCGACGCTGCCTCCGTGGGTGCCG |
| Internal bar code: | GAATTTTGGGACCCTCGCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 99.7765 |
| LEAP-Seq n confirming: | 893 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAACACTACACGCAGGC |
| Suggested primer 2: | GGTAATGTGGGCTGCTGTTT |