| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202649 |
| Chromosome: | chromosome 10 |
| Location: | 275655 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419700 | CGL6 | (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA); Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCAAGTGCTAGACAGGTGCAAACCTC |
| Internal bar code: | AGTGCTAAGCGGCGCTAACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 98.5603 |
| LEAP-Seq n confirming: | 2396 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGCTTCAAAAGCAGG |
| Suggested primer 2: | AACCCTAACACCACTGCCTG |