| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202660 |
| Chromosome: | chromosome 5 |
| Location: | 1166136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245550 | PIK1 | (1 of 1) PTHR10048//PTHR10048:SF15 - PHOSPHATIDYLINOSITOL KINASE // PHOSPHATIDYLINOSITOL 4-KINASE ALPHA-LIKE PROTEIN P2-RELATED; Phosphatidylinositol 4-kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGTAATTTAAGGAAGTGGTAGACGGTA |
| Internal bar code: | CTCTGCTGACCGGCTTTAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 99.3872 |
| LEAP-Seq n confirming: | 4703 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCTACCTGACCACAGGG |
| Suggested primer 2: | GCTGCTCCACATCTCAAACA |