| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.202666 |
| Chromosome: | chromosome 10 |
| Location: | 3239334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442950 | (1 of 2) PF13483 - Beta-lactamase superfamily domain (Lactamase_B_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATGGTAGTTACACCATTAATAGACGACC |
| Internal bar code: | ACGGTCGGTCCTGCGGGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 98.6741 |
| LEAP-Seq n confirming: | 1935 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCCGCTAGACTCGTTCT |
| Suggested primer 2: | GTGCCATACCTGACGTGTTG |