Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.202721 |
Chromosome: | chromosome_9 |
Location: | 3264327 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387912 | FAP139 | Flagellar Associated Protein, serine endopeptidase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGCGTAAATTCAAAGTGCACATGGCGTTA |
Internal bar code: | CATAAAAGGCGCGCGGGATCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 776 |
LEAP-Seq percent confirming: | 99.7302 |
LEAP-Seq n confirming: | 4436 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGGTTACACGATCACACG |
Suggested primer 2: | AGCTGAGGAGGAGAAGGAGG |